Dr Peter Thorpe
Senior Lecturer in Biochemistry
Deputy Dean for Research - Impact
School of Biological and Behavioural Sciences
Queen Mary University of London
Queen Mary University of London
Research
cell division, chromosome segregation, kinetochore, cell cycle
Interests
We perform research to understand the regulation of cell division. Cell division is the process that creates new cells to replace aged or damaged tissues in our body, however, uncontrolled cell division is the defining characteristic of cancer. We aim to determine how cell division is regulated to allow us to control this process. The ultimate aim is to be able to regulate cell division as a treatment for cancer and to promote cell division of stem cells for regenerative medicine.A central hypothesis of our work is that the position of proteins within the cell controls cell division. We place normal proteins in different locations within the cell, next to new partner proteins, and test the effect upon cell division. Some of the resulting changes promote cell division, while others block it. We then use this knowledge to understand both how the normal process of cell division is controlled and how it may be manipulated, for example in cancer therapy.
For more information see our lab website https://www.thorpelab.org
Publications
Publications of specific relevance to the Centre for Molecular Cell Biology2024
Proteome-wide forced interactions reveal a functional map of cell-cycle phospho-regulation in S. cerevisiaeKlemm C, Ólafsson G, Wood HR, Mellor C, Zabet NR and Thorpe PH
Nucleus, Taylor & Francis vol. 15 (1)
01-12-2024
Oncogenic c-Myc induces replication stress by increasing cohesins chromatin occupancy in a CTCF-dependent mannerPeripolli S, Meneguello L, Perrod C, Singh T, Patel H, Rahman ST, Kiso K, Thorpe P, Calvanese V, Bertoli C and de Bruin RAM
Nature Communications, Springer Nature vol. 15 (1), 1579-1579.
21-02-2024
2023
Constitutive methylation of budding yeast CENP-A contributes to chromosomal instabilityMishra PK, Ali N, Au W, Boeckmann L, Takahashi Y, Castineira P, Bloom K, Thorpe P and Basrai M
Molecular Biology of the Cell. vol. 34 (2), 964-964.
01-09-2023
Misregulation of cell cycle-dependent methylation of budding yeast CENP-A contributes to chromosomal instabilityMishra PK, Au W-C, Castineira PG, Ali N, Stanton J, Boeckmann L, Takahashi Y, Costanzo M, Boone C, Bloom KS, Thorpe PH and Basrai MA
Molecular Biology of The Cell, American Society For Cell Biology (Ascb) vol. 34 (10), ar99-ar99.
12-07-2023
2022
Comprehensive characterization of the Hsp70 interactome reveals novel client proteins and interactions mediated by posttranslational modifications.Nitika , Zheng B, Ruan L, Kline JT, Omkar S, Sikora J, Texeira Torres M, Wang Y, Takakuwa JE, Huguet R, Klemm C, Segarra VA, Winters MJ, Pryciak PM, Thorpe PH, Tatebayashi K, Li R, Fornelli L and Truman AW
Plos Biology, Public Library of Science vol. 20 (10), e3001839-e3001839.
21-10-2022
ScreenGarden: a shinyR application for fast and easy analysis of plate-based high-throughput screensKlemm C, Howell RSM and Thorpe PH
Bmc Bioinformatics, Springer Nature vol. 23 (1), 60-60.
05-02-2022
2021
Forced association of SARS-CoV-2 proteins with the yeast proteome perturb vesicle traffickingKlemm C, Wood H, Thomas GH, Ólafsson G, Torres MT and Thorpe PH
Microbial Cell, Shared Science Publishers Og vol. 8 (12), 280-280.
27-10-2021
Cdc7-mediated phosphorylation of Cse4 regulates high-fidelity chromosome segregation in budding yeast.Mishra PK, Wood H, Stanton J, Au W-C, Eisenstatt JR, Boeckmann L, Sclafani RA, Weinreich M, Bloom KS, Thorpe PH and Basrai MA
Molecular Biology of The Cell, American Society For Cell Biology (Ascb) vol. 32 (21), ar15-ar15.
25-08-2021
2020
Cell-cycle phospho-regulation of the kinetochoreKlemm C, Thorpe PH and Ólafsson G
Current Genetics, Springer Nature vol. 67 (2), 177-193.
22-11-2020
Unifying the mechanism of mitotic exit control in a spatiotemporal logical modelHowell RSM, Klemm C, Thorpe PH and Csikász-Nagy A
Plos Biology, Public Library of Science (Plos) vol. 18 (11)
12-11-2020
Asymmetric Transcription Factor Partitioning During Yeast Cell Division Requires the FACT Chromatin Remodeler and Cell Cycle Progression.Herrero E, Stinus S, Bellows E, Berry LK, Wood H and Thorpe PH
Genetics vol. 216 (3), 701-716.
01-11-2020
Polo kinase recruitment via the constitutive centromere-associated network at the kinetochore elevates centromeric RNAÓlafsson G and Thorpe PH
Plos Genetics, Public Library of Science (Plos) vol. 16 (8)
18-08-2020
CATS: Cas9-assisted tag switching. A high-throughput method for exchanging genomic peptide tags in yeastBerry LK, Thomas GH and Thorpe PH
Bmc Genomics, Biomed Central vol. 21 (1)
10-03-2020
FLIM, FRET and high content analysisGarcia E, Guo W, Kumar S, Görlitz F, Sparks H, Alexandrov Y, Munro I, Kelly DJ, Warren S, Chennell G, Sardini A, Carling D, Thorpe P, Dunsby C and French PMW
SPIE BiOS. vol. 11244
02-03-2020
2019
Synthetic Physical Interactions with the Yeast Centrosome.Howell RSM, Csikász-Nagy A and Thorpe PH
G3 (Bethesda) vol. 9 (7), 2183-2194.
09-07-2019
Synthetic Physical Interactions with the Yeast Centrosome.Howell RSM, Csikász-Nagy A and Thorpe PH
G3 (Bethesda) vol. 9 (7), 2183-2194.
01-07-2019
Cell cycle dependent association of polo kinase Cdc5 with CENP-A contributes to faithful chromosome segregation in budding yeast.Mishra PK, Olafsson G, Boeckmann L, Westlake TJ, Jowhar ZM, Dittman LE, Baker RE, D'Amours D, Thorpe PH and Basrai MA
Mol Biol Cell, mbcE18090584-mbcE18090584.
06-02-2019
Automated Fluorescence Lifetime Imaging High-Content Analysis of Förster Resonance Energy Transfer between Endogenously Labeled Kinetochore Proteins in Live Budding Yeast Cells.Guo W, Kumar S, Görlitz F, Garcia E, Alexandrov Y, Munro I, Kelly DJ, Warren S, Thorpe P, Dunsby C and French P
Slas Technol vol. 24 (3), 308-320.
10-01-2019
2017
Rewiring the Budding Yeast Proteome using Synthetic Physical InteractionsÓlafsson G and Thorpe PH
In Genome Instability, Springer Nature 599-612.
17-10-2017
2016
Bringing Functional Genomics into FocusLedesma-Fernández E, Thorpe PH and de Bruin RAM
Cell Systems, Elsevier Bv vol. 3 (3), 214-216.
01-09-2016
Synthetic Physical Interactions Map Kinetochore-Checkpoint Activation RegionsOlafsson G and Thorpe PH
G3-Genes Genomes Genetics vol. 6 (8), 2531-2542.
01-08-2016
Synthetic protein interactions reveal a functional map of the cellBerry LK, Ólafsson G, Ledesma-Fernández E and Thorpe PH
Elife, Elife vol. 5
21-04-2016
Synergistic Control of Kinetochore Protein Levels by Psh1 and Ubr2Herrero E and Thorpe PH
Plos Genetics, Public Library of Science (Plos) vol. 12 (2)
18-02-2016
2015
Synthetic physical interactions map kinetochore regulators and regions sensitive to constitutive Cdc14 localizationÓlafsson G and Thorpe PH
Proceedings of The National Academy of Sciences of The United States of America, Proceedings of The National Academy of Sciences vol. 112 (33), 10413-10418.
03-08-2015
Fluorescent foci quantitation for high-throughput analysis.Ledesma-Fernández E and Thorpe PH
Journal of Biological Methods, Journal of Biological Methods vol. 2 (2)
19-07-2015
2012
Visualizing global effects of the DNA damage responseThorpe PH and Rothstein R
Nature Cell Biology, Springer Nature vol. 14 (9), 900-901.
01-09-2012
ScreenTroll: a searchable database to compare genome-wide yeast screensThorpe PH, Dittmar JC and Rothstein R
Database, Oxford University Press (Oup) vol. 2012
01-01-2012
2011
Bringing Rad52 foci into focusThorpe PH, Alvaro D, Lisby M and Rothstein R
Journal of Cell Biology, Rockefeller University Press vol. 194 (5), 665-667.
05-09-2011
2009
Kinetochore asymmetry defines a single yeast lineageThorpe PH, Bruno J and Rothstein R
Proceedings of The National Academy of Sciences of The United States of America, Proceedings of The National Academy of Sciences vol. 106 (16), 6673-6678.
21-04-2009
2008
Modeling Stem Cell Asymmetry in YeastThorpe PH, Bruno J and Rothstein R
Cold Spring Harbor Symposia On Quantitative Biology, Cold Spring Harbor Laboratory vol. 73, 81-88.
01-01-2008
2007
More is not always better: the genetic constraints of polyploidyThorpe PH, González-Barrera S and Rothstein R
Trends in Genetics, Elsevier vol. 23 (6), 263-266.
05-04-2007
2006
Cells Expressing Murine RAD52 Splice Variants Favor Sister Chromatid RepairThorpe PH, Marrero VA, Savitzky MH, Sunjevaric I, Freeman TC and Rothstein R
Molecular and Cellular Biology, Taylor & Francis vol. 26 (10), 3752-3763.
01-05-2006
Functional genomics of the yeast DNA-damage responseCagney G, Alvaro D, Reid RJ, Thorpe PH, Rothstein R and Krogan NJ
Genome Biology, Springer Nature vol. 7 (9)
01-01-2006
2004
Towards Gene Therapy for Inflammatory and Infective Pulmonary DiseasesSimpson A, King J, Thorpe P, McLachlan G and Sallenave J-M
Current Genomics, Bentham Science Publishers vol. 5 (4), 365-383.
01-05-2004
2002
Optimising gene repair strategies in cell cultureThorpe P, Stevenson BJ and Porteous DJ
Gene Therapy, Springer Nature vol. 9 (11), 700-702.
22-05-2002
Functional correction of episomal mutations withshort DNA fragments and RNA–DNA oligonucleotidesThorpe PH, Stevenson BJ and Porteous DJ
The Journal of Gene Medicine, Wiley vol. 4 (2), 195-204.
01-03-2002
1999
Rapid Quantitation of Gene Therapy Specific CFTR Expression Using the Amplification Refractory Mutation SystemThorpe PH and Porteous DJ
Biotechniques, Taylor & Francis vol. 27 (1), 122-127.
01-07-1999
1997
The specificity of StySKI, a type I restriction enzyme, implies a structure with rotational symmetryThorpe PH, Ternent D and Murray NE
Nucleic Acids Research, Oxford University Press (Oup) vol. 25 (9), 1694-1700.
01-05-1997
The in Vitro Assembly of the EcoKI Type I DNA Restriction/Modification Enzyme and Its in Vivo Implications †Dryden DTF, Cooper LP, Thorpe PH and Byron O
Biochemistry, American Chemical Society (Acs) vol. 36 (5), 1065-1076.
01-02-1997
Grants
Grants of specific relevance to the Centre for Molecular Cell BiologyA confocal microscope for multidisciplinary dynamic studies of complex biological systemsConrad Mullineaux, Caroline Brennan, Alexandre De Mendoza Soler, Viji Draviam Sastry, Maurice Elphick, Christoph Engl, Isabel M Palacios, Matteo Palma, Marina Resmini, Alexander Ruban, Peter Thorpe, Angelika Stollewerk, Chema Martin Duran, Paulo Baptista Ribeiro, Susana Alves Godinho, Richard Grose, Stephanie Kermorgant, Peter McCormick and Tyson Sharp
£390,834 BBSRC Biotechnology and Biological Sciences Research Council
01-08-2022 - 31-07-2023
Super-Resolution Microscopy of live cells in 3DViji Draviam Sastry, Conrad Mullineaux, Vidya Chandran Darbari, Lakxmi Subramanian, Fabrice Caudron, Richard Pickersgill, Guy Hanke, Alexander Ruban, Peter Thorpe, Christopher Duffy, Chema Martin Duran, John Connelly, Peter McCormick, James Chapple, Vardhman Rakyan, Tyson Sharp, Susana Alves Godinho and David Lee
£369,201 BBSRC Biotechnology and Biological Sciences Research Council
01-12-2020 - 31-03-2022
The Francis Crick Institute transition grant award to Dr Peter ThorpePeter Thorpe
£384,000 Francis Crick Institute, The
01-04-2018 - 30-09-2022