Dr Charalampos (Babis) Rallis
Reader in Genetics, Genomics and Fundamental Cell Biology
Director of Industrial Innovation
School of Biological and Behavioural Sciences
Queen Mary University of London
Queen Mary University of London
Research
Ageing, Metabolism, Fission yeast, Neurons, Senescence, Phenomics
Interests
We study gene and protein networks implicated in cellular fitness and metabolism, neurodegeneration, cancer and ageing with a focus on the nutrient-responsive signalling pathway Target of Rapamycin (TOR).Our aim is to elucidate the molecular mechanisms and principles behind senescence and lifespan and apply this knowledge for the amelioration of age-related diseases. In addition, the group performs quantitative fitness profiling of microbiomes and mycobiomes and explore the effects of nutrition on biome physiology and subsequent effects on human healthy ageing.
We use the fission yeast Schizosaccharomyces pombe and mammalian 2D and 3D tissue culture systems and utilise multi-omics approaches and network biology.
Publications
2023
Islam RA and Rallis C (2023). Correction: Islam, R.A.; Rallis, C. Ribosomal Biogenesis and Heterogeneity in Development, Disease, and Aging. Epigenomes 2023, 7, 17. Epigenomes, MDPI vol. 7 (4) 26-10-2023
Gal C, Cochrane GA, Morgan BA, Rallis C, Bähler J and Whitehall SK (2023). The longevity and reversibility of quiescence in Schizosaccharomyces pombe are dependent upon the HIRA histone chaperone. Cell Cycle, Taylor & Francis vol. 22 (17), 1921-1936. 27-08-2023
Islam RA and Rallis C (2023). Ribosomal Biogenesis and Heterogeneity in Development, Disease, and Aging. Epigenomes, MDPI vol. 7 (3) 11-08-2023
Alao J-P, Legon L, Dabrowska A, Tricolici A-M, Kumar J and Rallis C (2023). Interplays of AMPK and TOR in Autophagy Regulation in Yeast. Cells, MDPI vol. 12 (4) 04-02-2023
2022
Dabrowska A, Kumar J and Rallis C (2022). Nutrient-Response Pathways in Healthspan and Lifespan Regulation. Cells, MDPI vol. 11 (9) 06-05-2022
2021
Martinez-Miguel VE, Lujan C, Espie--Caullet T, Martinez-Martinez D, Moore S, Backes C, Gonzalez S, Galimov ER, Brown AEX, Halic M, Tomita K, Rallis C, von der Haar T, Cabreiro F and Bjedov I (2021). Increased fidelity of protein synthesis extends lifespan. Cell Metabolism, Elsevier vol. 33 (11), 2288-2300.e12. 14-09-2021
Legon L and Rallis C (2021). Genome-wide screens in yeast models towards understanding chronological lifespan regulation. Briefings in Functional Genomics, Oxford University Press (OUP) vol. 21 (1), 4-12. 17-03-2021
Alao J-P, Legon L and Rallis C (2021). Crosstalk between the mTOR and DNA Damage Response Pathways in Fission Yeast. Cells, MDPI vol. 10 (2) 02-02-2021
2020
Rallis C, Mülleder M, Smith G, Au YZ, Ralser M and Bähler J (2020). Amino Acids Whose Intracellular Levels Change Most During Aging Alter Chronological Life Span of Fission Yeast. The Journals of Gerontology Series A, Oxford University Press (OUP) vol. 76 (2), 205-210. 03-11-2020
Alao JP, Johansson-Sjölander J, Rallis C and Sunnerhagen P (2020). Caffeine Stabilises Fission Yeast Wee1 in a Rad24-Dependent Manner but Attenuates Its Expression in Response to DNA Damage. Microorganisms, MDPI vol. 8 (10) 30-09-2020
Bjedov I and Rallis C (2020). The Target of Rapamycin Signalling Pathway in Ageing and Lifespan Regulation. Genes, MDPI vol. 11 (9) 03-09-2020
Rodríguez-López M, Gonzalez S, Hillson O, Tunnacliffe E, Codlin S, Tallada VA, Bähler J and Rallis C (2020). The GATA Transcription Factor Gaf1 Represses tRNAs, Inhibits Growth, and Extends Chronological Lifespan Downstream of Fission Yeast TORC1. Cell Reports, Elsevier vol. 30 (10), 3240-3249.e4. 01-03-2020
2019
Pai C-C, Hsu K-F, Durley SC, Keszthelyi A, Kearsey SE, Rallis C, Folkes LK, Deegan R, Wilkins SE, Pfister SX, De León N, Schofield CJ, Bähler J, Carr AM and Humphrey TC (2019). An essential role for dNTP homeostasis following CDK-induced replication stress. Journal of Cell Science, The Company of Biologists vol. 132 (6) 15-03-2019
2018
Hillson O, Gonzalez S and Rallis C (2018). Prospects of Pharmacological Interventions to Organismal Aging. BioMolecular Concepts, De Gruyter vol. 9 (1), 200-215. 31-12-2018
Atkinson SR, Marguerat S, Bitton DA, Rodríguez-López M, Rallis C, Lemay J-F, Cotobal C, Malecki M, Smialowski P, Mata J, Korber P, Bachand F and Bähler J (2018). Long noncoding RNA repertoire and targeting by nuclear exosome, cytoplasmic exonuclease, and RNAi in fission yeast. RNA, Cold Spring Harbor Laboratory vol. 24 (9), 1195-1213. 18-06-2018
Ellis DA, Mustonen V, Rodríguez-López M, Rallis C, Malecki M, Jeffares DC and Bähler J (2018). Uncovering Natural Longevity Alleles from Intercrossed Pools of Aging Fission Yeast Cells. Genetics, Oxford University Press (OUP) vol. 210 (2), 733-744. 08-02-2018
2017
Hoyos-Manchado R, Reyes-Martín F, Rallis C, Gamero-Estévez E, Rodríguez-Gómez P, Quintero-Blanco J, Bähler J, Jiménez J and Tallada VA (2017). RNA metabolism is the primary target of formamide in vivo. Scientific Reports, Springer Nature vol. 7 (1) 21-11-2017
Rallis C, Townsend S and Bähler J (2017). Erratum: Corrigendum: Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes. Scientific Reports, Springer Nature vol. 7 (1) 10-07-2017
Rallis C, Townsend S and Bähler J (2017). Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes. Scientific Reports, Springer Nature vol. 7 (1) 10-03-2017
Jeffares DC, Jolly C, Hoti M, Speed D, Shaw L, Rallis C, Balloux F, Dessimoz C, Bähler J and Sedlazeck FJ (2017). Transient structural variations have strong effects on quantitative traits and reproductive isolation in fission yeast. Nature Communications, Springer Nature vol. 8 (1) 24-01-2017
Gonzalez S and Rallis C (2017). The TOR Signaling Pathway in Spatial and Temporal Control of Cell Size and Growth. Front Cell Dev Biol vol. 5 01-01-2017
2016
Malecki M, Bitton DA, Rodríguez-López M, Rallis C, Calavia NG, Smith GC and Bähler J (2016). Functional and regulatory profiling of energy metabolism in fission yeast. Genome Biology, Springer Nature vol. 17 (1) 25-11-2016
Brault A, Rallis C, Normant V, Garant J-M, Bähler J and Labbé S (2016). Php4 Is a Key Player for Iron Economy in Meiotic and Sporulating Cells. G3: Genes, Genomes, Genetics, Oxford University Press (OUP) vol. 6 (10), 3077-3095. 01-10-2016
Bischof L, Pevorovsk M, Rallis C, Jeffares DC, Arzhaeva Y and Bhler J (2016). Spotsizer: High-throughput quantitative analysis of microbial growth. BioTechniques, Taylor & Francis vol. 61 (4), 191-201. 01-10-2016
Vishwanatha A, Rallis C, Subramanyaswamy SB, D'Souza CJM, Bähler J and Schweingruber ME (2016). Identification of nuclear genes affecting 2-Deoxyglucose resistance in Schizosaccharomyces pombe. FEMS Yeast Research, Oxford University Press (OUP) vol. 16 (6) 31-07-2016
Tomalin LE, Day AM, Underwood ZE, Smith GR, Pezze PD, Rallis C, Patel W, Dickinson BC, Bähler J, Brewer TF, Chang CJ-L, Shanley DP and Veal EA (2016). Increasing extracellular H2O2 produces a bi-phasic response in intracellular H2O2, with peroxiredoxin hyperoxidation only triggered once the cellular H2O2-buffering capacity is overwhelmed. Free Radical Biology and Medicine, Elsevier vol. 95, 333-348. 02-03-2016
2015
Bond ME, Brown R, Rallis C, Bähler J and Mole SE (2015). A central role for TOR signalling in a yeast model for juvenile CLN3 disease. Microbial Cell, Shared Science Publishers OG vol. 2 (12) 07-12-2015
Rallis C and Bähler J (2015). Cell-based screens and phenomics with fission yeast. Critical Reviews in Biochemistry and Molecular Biology, Taylor & Francis vol. 51 (2), 86-95. 02-11-2015
Bitton DA, Atkinson SR, Rallis C, Smith GC, Ellis DA, Chen YYC, Malecki M, Codlin S, Lemay J-F, Cotobal C, Bachand F, Marguerat S, Mata J and Bähler J (2015). Widespread exon skipping triggers degradation by nuclear RNA surveillance in fission yeast. Genome Research, Cold Spring Harbor Laboratory vol. 25 (6), 884-896. 16-04-2015
Jeffares DC, Rallis C, Rieux A, Speed D, Převorovský M, Mourier T, Marsellach FX, Iqbal Z, Lau W, Cheng TMK, Pracana R, Mülleder M, Lawson JLD, Chessel A, Bala S, Hellenthal G, O'Fallon B, Keane T, Simpson JT, Bischof L, et al. (2015). The genomic and phenotypic diversity of Schizosaccharomyces pombe. Nature Genetics, Springer Nature vol. 47 (3), 235-241. 09-02-2015
Sideri T, Rallis C, Bitton DA, Lages BM, Suo F, Rodríguez-López M, Du L-L and Bähler J (2015). Parallel Profiling of Fission Yeast Deletion Mutants for Proliferation and for Lifespan During Long-Term Quiescence. G3: Genes, Genomes, Genetics, Oxford University Press (OUP) vol. 5 (1), 145-155. 01-01-2015
2014
Sofola-Adesakin O, Castillo-Quan JI, Rallis C, Tain LS, Bjedov I, Rogers I, Li L, Martinez P, Khericha M, Cabecinha M, Bähler J and Partridge L (2014). Lithium suppresses Aβ pathology by inhibiting translation in an adult Drosophila model of Alzheimer's disease. Frontiers in Aging Neuroscience, Frontiers vol. 6 30-07-2014
Bitton DA, Rallis C, Jeffares DC, Smith GC, Chen YYC, Codlin S, Marguerat S and Bähler J (2014). LaSSO, a strategy for genome-wide mapping of intronic lariats and branch points using RNA-seq. Genome Research, Cold Spring Harbor Laboratory vol. 24 (7), 1169-1179. 07-04-2014
Rallis C, López-Maury L, Georgescu T, Pancaldi V and Bähler J (2014). Systematic screen for mutants resistant to TORC1 inhibition in fission yeast reveals genes involved in cellular ageing and growth. Biology Open, The Company of Biologists vol. 3 (2), 161-171. 17-01-2014
2013
Rallis C and Bähler J (2013). Inhibition of TORC1 signaling and increased lifespan: gained in translation? Aging, Impact Journals vol. 5 (5), 335-336. 21-05-2013
Rallis C, Codlin S and Bähler J (2013). TORC1 signaling inhibition by rapamycin and caffeine affect lifespan, global gene expression, and cell proliferation of fission yeast. Aging Cell, Wiley vol. 12 (4), 563-573. 02-05-2013
2012
Pancaldi V, Saraç ÖS, Rallis C, McLean JR, Převorovský M, Gould K, Beyer A and Bähler J (2012). Predicting the Fission Yeast Protein Interaction Network. G3: Genes, Genomes, Genetics, Oxford University Press (OUP) vol. 2 (4), 453-467. 01-04-2012
2011
2010
Rallis C, Pinchin SM and Ish-Horowicz D (2010). Cell-autonomous integrin control of Wnt and Notch signalling during somitogenesis. Development, The Company of Biologists vol. 137 (21), 3591-3601. 28-09-2010
Maydan M, McDonald PC, Sanghera J, Yan J, Rallis C, Pinchin S, Hannigan GE, Foster LJ, Ish-Horowicz D, Walsh MP and Dedhar S (2010). Integrin-Linked Kinase Is a Functional Mn2+-Dependent Protein Kinase that Regulates Glycogen Synthase Kinase-3β (GSK-3β) Phosphorylation. PLOS ONE, Public Library of Science (PLoS) vol. 5 (8) 23-08-2010
2009
2008
2007
2006
2005
Rallis C, Del Buono J and Logan MPO (2005). Tbx3 can alter limb position along the rostrocaudal axis of the developing embryo. Development, The Company of Biologists vol. 132 (8), 1961-1970. 15-04-2005
2003
Rallis C, Bruneau BG, Del Buono J, Seidman CE, Seidman JG, Nissim S, Tabin CJ and Logan MPO (2003). Tbx5 is required for forelimb bud formation and continued outgrowth. Development, The Company of Biologists vol. 130 (12), 2741-2751. 15-06-2003
2001
Rallis C, Stamataki D, Pontikakis S, Mankoo BS and Karagogeos D (2001). Isolation of the avian homologue of the homeobox gene Mox2 and analysis of its expression pattern in developing somites and limbs. Cells and Development, Elsevier vol. 104 (1-2), 121-124. 01-06-2001
Grants
Integrating cellular space and time: inteplays between subcellular organisation and lifespan-GRANT TRANSFERCharalampos (Babis) Rallis
£269,451 BBSRC Biotechnology and Biological Sciences Research Council (01-09-2023 - 14-04-2026)
Mechanisms of uncoupling cellular growth from mTOR pathway activity - GRANT TRANSFERCharalampos (Babis) Rallis
£292,263 MRC Medical Research Council (01-09-2023 - 13-02-2025)