Research
Artificial Cell, Polymersome, Polymer Chemistry, Synthetic Biology, Nanotechnology
Interests
I work at the interface of “blue skies” research and technology development in the biomimetic construction of artificial cells. Artificial cells are finding increasing importance in biotechnology, therapy, and diagnostics. Hence, methods to expand or improve the function of living cells by artificial systems are urgently needed. My group is currently finding mechanisms that can modulate the biophysical properties of membranes such as their rigidity, elastic moduli, and their ability to respond to stimuli to a) understand and exploit membrane-associated processes and b) to produce membrane materials with novel functionality (responsiveness to light, hydromechanical stress, pH, magnetism, gasses, enzymes, redox potentials, etc.). My approach involves the introduction of polymers that present enhanced biomolecular versatility compared to state-of-the-art building blocks in artificial cell research. The control of their architecture and composition can generate combinations that resemble protein and nucleic acid versatility.
Publications

Publications of specific relevance to the Centre for Chemical Research
2024
Unlocking Intracellular Protein Delivery by Harnessing Polymersomes Synthesized at Microliter Volumes using Photo‐PISAThanapongpibul C, Rifaie‐Graham O, Ojansivu M, Najer A, Kim H, Bakker SE, Chami M, Peeler DJ, Liu C, Yeow J and Stevens MM
Advanced Materials,
Wiley vol. 36 (49)
17-10-20242023
Emergence of Cell Behavior Through Feedback Control of Polymersome Membrane TransportRifaie‐Graham O
Chemsystemschem,
Wiley vol. 6 (1)
05-12-2023
Assembly of Fillable Microrobotic Systems by Microfluidic Loading with Dip SealingSun R, Song X, Zhou K, Zuo Y, Wang R, Rifaie‐Graham O, Peeler DJ, Xie R, Leng Y, Geng H, Brachi G, Ma Y, Liu Y, Barron L and Stevens MM
Advanced Materials,
Wiley vol. 35 (13), e2207791-e2207791.
26-02-2023
Visible light-responsive materials: the (photo)chemistry and applications of donor–acceptor Stenhouse adducts in polymer scienceClerc M, Sandlass S, Rifaie-Graham O, Peterson JA, Bruns N, de Alaniz JR and Boesel LF
Chemical Society Reviews,
Royal Society of Chemistry (Rsc) vol. 52 (23), 8245-8294.
01-01-20232022
Differences in Human Plasma Protein Interactions between Various Polymersomes and Stealth Liposomes as Observed by Fluorescence Correlation SpectroscopyNajer A, Rifaie‐Graham O, Yeow J, Adrianus C, Chami M and Stevens MM
Macromolecular Bioscience,
Wiley vol. 23 (8), e2200424-e2200424.
16-12-2022
Photoswitchable gating of non-equilibrium enzymatic feedback in chemically communicating polymersome nanoreactorsRifaie-Graham O, Yeow J, Najer A, Wang R, Sun R, Zhou K, Dell TN, Adrianus C, Thanapongpibul C, Chami M, Mann S, de Alaniz JR and Stevens MM
Nature Chemistry,
Springer Nature vol. 15 (1), 110-118.
07-11-2022
Microliter Scale Synthesis of Luciferase‐Encapsulated Polymersomes as Artificial Organelles for Optogenetic Modulation of Cardiomyocyte BeatingKim H, Yeow J, Najer A, Kit‐Anan W, Wang R, Rifaie‐Graham O, Thanapongpibul C and Stevens MM
Advanced Science,
Wiley vol. 9 (27)
28-07-2022
Potent Virustatic Polymer–Lipid Nanomimics Block Viral Entry and Inhibit Malaria Parasites In VivoNajer A, Blight J, Ducker CB, Gasbarri M, Brown JC, Che J, Høgset H, Saunders C, Ojansivu M, Lu Z, Lin Y, Yeow J, Rifaie-Graham O, Potter M, Tonkin R, Penders J, Doutch JJ, Georgiadou A, Barriga HMG, Holme MN,
et al. Acs Central Science,
American Chemical Society (Acs) vol. 8 (9), 1238-1257.
03-05-20222020
Shear Stress‐Responsive Polymersome Nanoreactors Inspired by the Marine Bioluminescence of DinoflagellatesRifaie‐Graham O, Galensowske NFB, Dean C, Pollard J, Balog S, Gouveia MG, Chami M, Vian A, Amstad E, Lattuada M and Bruns N
Angewandte Chemie,
Wiley vol. 133 (2), 917-922.
12-11-2020
Rapid quantification of the malaria biomarker hemozoin by improved biocatalytically initiated precipitation atom transfer radical polymerizationsRaccio S, Pollard J, Djuhadi A, Balog S, Pellizzoni MM, Rodriguez KJ, Rifaie-Graham O and Bruns N
Analyst,
Royal Society of Chemistry (Rsc) vol. 145 (23), 7741-7751.
01-01-20202019
Biocatalytically Initiated Precipitation Atom Transfer Radical Polymerization (ATRP) as a Quantitative Method for Hemoglobin Detection in Biological FluidsPollard J, Rifaie-Graham O, Raccio S, Davey A, Balog S and Bruns N
Analytical Chemistry,
American Chemical Society (Acs) vol. 92 (1), 1162-1170.
02-12-2019
Hemozoin-catalyzed precipitation polymerization as an assay for malaria diagnosisRifaie-Graham O, Pollard J, Raccio S, Balog S, Rusch S, Hernández-Castañeda MA, Mantel P-Y, Beck H-P and Bruns N
Nature Communications,
Springer Nature vol. 10 (1)
25-03-2019
Bio-Inspired Polymersome NanoreactorsRifaie-Graham O, Apebende EA and Bruns N
Chimia International Journal For Chemistry,
Swiss Chemical Society vol. 73 (1), 21-24.
27-02-20192018
The Kinetics of β‐Hematin Crystallization Measured by Depolarized Light ScatteringRifaie‐Graham O, Hua X, Bruns N and Balog S
Small,
Wiley vol. 14 (46)
03-09-2018
Wavelength-Selective Light-Responsive DASA-Functionalized Polymersome NanoreactorsRifaie-Graham O, Ulrich S, Galensowske NFB, Balog S, Chami M, Rentsch D, Hemmer JR, de Alaniz JR, Boesel LF and Bruns N
Journal of The American Chemical Society,
American Chemical Society (Acs) vol. 140 (25), 8027-8036.
01-06-2018
Self‐Reporting Fiber‐Reinforced Composites That Mimic the Ability of Biological Materials to Sense and Report DamageRifaie‐Graham O, Apebende EA, Bast LK and Bruns N
Advanced Materials,
Wiley vol. 30 (19)
24-03-20182016
Laccase-catalyzed controlled radical polymerization of N -vinylimidazoleFodor C, Gajewska B, Rifaie-Graham O, Apebende EA, Pollard J and Bruns N
Polymer Chemistry,
Royal Society of Chemistry (Rsc) vol. 7 (43), 6617-6625.
01-01-2016