Prof Steffi Krause

Dr. rer. nat., MRSC

Professor of Electroanalytical Systems

School of Engineering and Materials Science
Queen Mary University of London

s.krause@qmul.ac.uk

www.sems.qmul.ac.uk/staff/s.krause/

Centre for Bioengineering

Research
Publications
Grants

Research

Photoelectrochemical imaging, Light-addressable potentiometric sensors, Light-activated electrochemistry, Biosensors, Live-cell imaging, Materials Science

Interests

My research focuses on the development of chemical and biological sensors and electrochemical imaging. Also visit our group web page: https://www.sems.qmul.ac.uk/research/groups/electrochemic...

Light-Addressable Potentiometric Sensors (LAPS) and Scanning Photo-induced Impedance Microscopy (SPIM) are imaging techniques based on photocurrent measurements at electrolyte/insulator/silicon (EIS) field-effect structures. They are capable of measuring local changes in the surface potential (LAPS), which can be used to image ion concentrations, and of measuring the local impedance (SPIM). Recent highlights of our research are the achievement of submicron resolution of photocurrent measurements at silicon-on-sapphire substrates using a two-photon effect, the increase in the sensitivity of SPIM using organic, self-assembled monolayers as insulators, the use of SPIM for the interrogation of biosensor arrays for the detection of charged molecules such as DNA, quantitative impedance imaging of polyelectrolyte microcapsules with high resolution and the measurement of cell surface charge and cell impedance.

Recent development of photoelectrochemical imaging (PEI) with semiconducting metal oxide substrates such as indium tin oxide and hematite allows detection of redox active molecules and hence a much broader range of analytes than with traditional EIS structures. Combined with a recently developed fast imaging setup, the technique has shown great potential for continuous functional electrochemical imaging of the attachment area of cells, providing information such as ion concentration, action potentials and cell membrane permeability. Photoelectrochemical imaging is particularly attractive for label-free in-situ monitoring in in-vitro models.

Publications

Publications of specific relevance to the Centre for Bioengineering

2024

Actively Driven Light-Addressable Sensor/Actuator System for Automated pH Control for the Integration in Lab-On-A-Chip (LoC) Platforms
Welden R, Das A, Krause S, Schöning MJ, Wagner PH and Wagner T
Acs Sensors, American Chemical Society (Acs) vol. 9 (3), 1533-1544.

DOI 10.1021/acssensors.3c02712

QMRO

06-03-2024

2023

Numerical investigation of a graphene-on-semiconductor device for optical monitoring of cell electrophysiology
Gorecki J and Krause S
Iscience, Elsevier vol. 27 (1)

DOI 10.1016/j.isci.2023.108554

QMRO

24-11-2023

2022

Photoelectrochemical imaging of single cardiomyocytes and monitoring of their action potentials through contact force manipulation of organoids
Jacques R, Zhou B, Marhuenda E, Gorecki J, Das A, Iskratsch T and Krause S
Biosensors and Bioelectronics, Elsevier

DOI 10.1016/j.bios.2022.115024

QMRO

21-12-2022

High-resolution impedance and potential imaging using light-addressable potentiometric sensors for bio-imaging applications
Krause S
, 222-222.

DOI 10.11470/jsapmeeting.2017.1.0_222

23-11-2022

Photoelectrochemical Detection of Calcium Ions Based on Hematite Nanorod Sensors
Zhou B, Jiang Y, Guo Q, Das A, Jorge Sobrido A, Hing K, Zayats A and Krause S
Acs Applied Nano Materials, American Chemical Society

DOI 10.1021/acsanm.2c03978

QMRO

10-11-2022

Light-Addressable Electrochemical Sensors toward Spatially Resolved Biosensing and Imaging Applications
Meng Y, Chen F, Wu C, Krause S, Wang J and Zhang DW
Acs Sensors, American Chemical Society

DOI 10.1021/acssensors.2c00940

QMRO

28-06-2022

2021

Self-powered ultrasensitive and highly stretchable temperature–strain sensing composite yarns
Wan K, Liu Y, Santagiuliana G, Barandun G, Taroni Junior P, Güder F, Bastiaansen CWM, Baxendale M, Fenwick O, Papageorgiou DG, Krause S, Zhang H and Bilotti E
Materials Horizons

DOI 10.1039/d1mh00908g

QMRO

12-07-2021

Photoelectrochemical imaging system with high spatiotemporal resolution for visualizing dynamic cellular responses
Zhou B, Das A, Zhong M, Guo Q, Zhang D-W, Hing KA, Sobrido AJ, Titirici M-M and Krause S
Biosensors and Bioelectronics, Elsevier vol. 180

DOI 10.1016/j.bios.2021.113121

QMRO

02-03-2021

2020

Ammonia Gas Sensor Response of a Vertical Zinc Oxide Nanorod-Gold Junction Diode at Room Temperature
Tu Y, Kyle C, Luo H, Zhang DW, Das A, Briscoe J, Dunn S, Titirici MM and Krause S
Acs Sensors vol. 5 (11), 3568-3575.

DOI 10.1021/acssensors.0c01769

QMRO

25-11-2020

2019

InGaN as a substrate for AC photoelectrochemical imaging
Zhou B, Das A, Kappers M, Oliver R, Humphreys C and Krause S
Sensors, Mdpi

DOI 10.3390/s19204386

QMRO

11-10-2019

Modulated light-activated electrochemistry at silicon functionalized with metal-organic frameworks towards addressable DNA chips.
Wang J, Yang Z, Chen W, Du L, Jiao B, Krause S, Wang P, Wei Q, Zhang D-W and Wu C
Biosensors and Bioelectronics, Elsevier vol. 146, 111750-111750.

DOI 10.1016/j.bios.2019.111750

QMRO

03-10-2019

Flexible and Stretchable Self‐Powered Multi‐Sensors Based on the N‐Type Thermoelectric Response of Polyurethane/Na x (Ni‐ett) n Composites
Wan K, Taroni PJ, Liu Z, Liu Y, Tu Y, Santagiuliana G, Hsia I, Zhang H, Fenwick O, Krause S, Baxendale M, Schroeder BC and Bilotti E
Advanced Electronic Materials, Wiley, 1900582-1900582.

DOI 10.1002/aelm.201900582

QMRO

15-09-2019

Peptide Cross-Linked Poly(2-oxazoline) as a Sensor Material for the Detection of Proteases with a Quartz Crystal Microbalance.
Ahmad N, Colak B, Gibbs MJ, Zhang D-W, Gautrot JE, Watkinson M, Becer CR and Krause S
Biomacromolecules vol. 20 (7), 2506-2514.

DOI 10.1021/acs.biomac.9b00245

QMRO

07-06-2019

Photoelectrochemical Imaging System for the Mapping of Cell Surface Charges.
Wu F, Zhou B, Wang J, Zhong M, Das A, Watkinson M, Hing K, Zhang D-W and Krause S
Anal Chem

DOI 10.1021/acs.analchem.9b00304

QMRO

15-04-2019

Peptide Cross-Linked Poly (Ethylene Glycol) Hydrogel Films as Biosensor Coatings for the Detection of Collagenase
Ahmad N, Colak B, Zhang D-W, Gibbs MJ, Watkinson M, Becer CR, Gautrot JE and Krause S
Sensors, Mdpi vol. 19 (7)

DOI 10.3390/s19071677

QMRO

08-04-2019

2018

Photoelectrochemical Imaging Using Carbon Dots (CDs) Derived from Chitosan
Zhang D, Papaioannou N, Titirici M-M and Krause S
Proceedings, Mdpi vol. 2 (13)

DOI 10.3390/proceedings2130778

10-12-2018

Collagenase Biosensor Based on the Degradation of Peptide Cross-Linked Poly(Ethylene Glycol) Hydrogel Films
Ahmad N, Colak B, Gibbs MJ, Zhang D-W, Becer CR, Watkinson M, Gautrot JE and Krause S
, Mdpi vol. 2 (13)

DOI 10.3390/proceedings2130961

QMRO

30-11-2018

The Effect of Semiconductor Morphology on the Spatial Resolution of ZnO Based Light-Addressable Potentiometric Sensors
Tu Y, Li J, Zhang D-W, Briscoe J and Krause S
EUROSENSORS 2018. vol. 2 (13)

DOI 10.3390/proceedings2130917

27-11-2018

Light-Addressable Potentiometric Sensors using ZnO Nanorods as the Sensor Substrate for Bioanalytical Applications
TU Y, AHMAD N, BRISCOE J, ZHANG D and KRAUSE S
Analytical Chemistry, American Chemical Society

DOI 10.1021/acs.analchem.8b02244

QMRO

22-06-2018

Surface modification and construction of LAPS towards biosensing applications
Wang J, Du L, Krause S, Wu C and Wang P
Sensors and Actuators, B: Chemical vol. 265, 161-173.

DOI 10.1016/j.snb.2018.02.190

QMRO

08-03-2018

Photoelectrochemical response of carbon dots (CDs) derived from chitosan and their use in electrochemical imaging
ZHANG D, PAPAIOANNOU N, David NM, LUO H, GAO H, Tanase LC, Degousee T, Samori P, SAPELKIN A, FENWICK O, TITIRICI M and KRAUSE SK
Materials Horizons, Rsc

DOI 10.1039/c7mh00784a

QMRO

12-02-2018

Control of oxygen vacancies in ZnO nanorods by annealing and their influence on ZnO/PEDOT:PSS diode behaviour
TU Y, CHEN S, LI X, GORBACIOVA J, GILLIN WP, KRAUSE S and BRISCOE J
Journal of Materials Chemistry C, Royal Society of Chemistry

DOI 10.1039/C7TC04284A

QMRO

02-02-2018

Light-Addressable Potentiometric Sensors for (Bio-)chemical Sensing and Imaging
Schöning MJ, Wagner T, Poghossian A, Miyamoto K-I, Werner CF, Krause S and Yoshinobu T
In Encyclopedia of Interfacial Chemistry, Elsevier 295-308.

DOI 10.1016/b978-0-12-409547-2.13483-3

01-01-2018

(Bio-)chemical Sensing and Imaging by LAPS and SPIM
Yoshinobu T, Krause S, Miyamoto K-I, Werner CF, Poghossian A, Wagner T and Schöning MJ
In Label-Free Biosensing, Springer Nature 103-132.

DOI 10.1007/5346_2017_22

01-01-2018

2017

LAPS and SPIM imaging using ITO coated glass as the substrate material
Zhang D, Wu F and KRAUSE SK
Analytical Chemistry, American Chemical Society

DOI 10.1021/acs.analchem.7b01898

QMRO

05-07-2017

Biological imaging using light-addressable potentiometric sensors and scanning photo-induced impedance microscopy
Wu F, Campos I, Zhang D and KRAUSE SK
Proceedings of The Royal Society a: Mathematical, Physical and Engineering Sciences, Royal Society, The

DOI 10.1098/rspa.2017.0130

QMRO

03-05-2017

Image Detection of Yeast Saccharomyces Cerevisiae By Light-Addressable Potentiometric Sensors (LAPS)
Zhang D and Krause S
Ecs Meeting Abstracts, The Electrochemical Society vol. MA2017-01 (38), 1770-1770.

DOI 10.1149/ma2017-01/38/1770

15-04-2017

Electrochemical Imaging of Copper Contamination on Self-Assembled Organic Monolayer Modified Silicon Surfaces Following a ‘Click’ Reaction with Light-Addressable Potentiometric Sensors and Scanning Photo-Induced Impedance Microscopy
Wu F, Watkinson M and Krause S
Ecs Meeting Abstracts, The Electrochemical Society vol. MA2017-01 (38), 1769-1769.

DOI 10.1149/ma2017-01/38/1769

15-04-2017

Copper Contamination of Self-Assembled Organic Monolayer Modified Silicon Surfaces Following a “Click” Reaction Characterized with LAPS and SPIM
Wu F, Zhang DW, Wang J, Watkinson M and Krause S
Langmuir vol. 33 (13), 3170-3177.

DOI 10.1021/acs.langmuir.6b03831

QMRO

04-04-2017

2016

Image detection of yeast Saccharomyces cerevisiae by light-addressable potentiometric sensors (LAPS)
Zhang DW, Wu F, Wang J, Watkinson M and KRAUSE SK
Electrochemistry Communications vol. 72, 41-45.

DOI 10.1016/j.elecom.2016.08.017

QMRO

24-08-2016

The effect of gold nanoparticles on the impedance of microcapsules visualized by scanning photo-induced impedance microscopy
Wang J, Campos I, Wu F, Zhu J, Sukhorukov GB, Palma M, Watkinson M and Krause S
Electrochimica Acta vol. 208, 39-46.

DOI 10.1016/j.electacta.2016.05.017

QMRO

04-05-2016

2015

click Patterning of Self-Assembled Monolayers on Hydrogen-Terminated Silicon Surfaces and Their Characterization Using Light-Addressable Potentiometric Sensors
Wang J, Wu F, Watkinson M, Zhu J and Krause S
Langmuir vol. 31 (35), 9646-9654.

DOI 10.1021/acs.langmuir.5b02069

QMRO

08-09-2015

α-Amylase sensor based on the degradation of oligosaccharide hydrogel films monitored with a quartz crystal sensor.
Gibbs MJ, Biela A and Krause S
Biosens Bioelectron vol. 67, 540-545.

DOI 10.1016/j.bios.2014.09.036

QMRO

15-05-2015

Incorporation of Ag nanowires in CuWO4 for improved visible light-induced photoanode performance
Zhang H, Yilmaz P, Ansari JO, Khan FF, Binions R, Krause S and Dunn S
Journal of Materials Chemistry A vol. 3 (18), 9638-9644.

DOI 10.1039/c4ta07213h

QMRO

26-03-2015

Disposable MMP-9 sensor based on the degradation of peptide cross-linked hydrogel films using electrochemical impedance spectroscopy
Biela A, Watkinson M, Meier UC, Baker D, Giovannoni G, Becer CR and Krause S
Biosensors and Bioelectronics vol. 68, 660-667.

DOI 10.1016/j.bios.2015.01.060

QMRO

27-01-2015

2014

High-sensitivity light-addressable potentiometric sensors using silicon on sapphire functionalized with self-assembled organic monolayers
Wang J, Zhou Y, Watkinson M, Gautrot J and Krause S
Sensors and Actuators B: Chemical, Elsevier vol. 209, 230-236.

DOI 10.1016/j.snb.2014.11.071

QMRO

26-11-2014

2013

Degradation behaviour of thin polymer films of poly (amide ester) hydrogel using quartz crystal microbalance
Dzaraini K, Norizan A, Krause S, Hunter C and Milanesi L
Advanced Materials Research vol. 812, 38-45.

DOI 10.4028/www.scientific.net/AMR.812.38

29-10-2013

2012

A label-free aptasensor for the sensitive and specific detection of cocaine using supramolecular aptamer fragments/target complex by electrochemical impedance spectroscopy
Zhang DW, Zhang FT, Cui YR, Deng QP, Krause S, Zhou YL and Zhang XX
Talanta vol. 92, 65-71.

DOI 10.1016/j.talanta.2012.01.049

15-04-2012

2011

Generic protease detection technology for monitoring periodontal disease
Zheng X, Cook JP, Watkinson M, Yang S, Douglas I, Rawlinson A and Krause S
Faraday Discussions vol. 149, 37-47.

DOI 10.1039/c005364c

17-01-2011

2010

High resolution LAPS and SPIM
Chen L, Zhou Y, Jiang S, Kunze J, Schmuki P and Krause S
Electrochemistry Communications vol. 12 (6), 758-760.

DOI 10.1016/j.elecom.2010.03.026

01-06-2010

2009

A peptide cross-linked polyacrylamide hydrogel for the detection of human neutrophil elastase
Kamarun D, Zheng XW, Milanesi L, Hunter CA and Krause S
Electrochimica Acta vol. 54 (22), 4985-4990.

DOI 10.1016/j.electacta.2009.03.067

QMRO

01-09-2009

Sensor materials for the detection of proteases
Stair JL, Watkinson M and Krause S
Biosensors and Bioelectronics vol. 24 (7), 2113-2118.

DOI 10.1016/j.bios.2008.11.002

15-03-2009

Selected Papers from the Second International Conference on Optical, Optoelectronic and Photonic Materials and Applications, 2007
Sweeney SJ and Krause S
J Mater Sci-Mater El vol. 20, 1-2.

DOI 10.1007/s10854-008-9740-z

01-01-2009

Editorial: Selected papers from the second international conference on optical, optoelectronic and photonic materials and applications, 2007
Sweeney SJ and Krause S
Journal of Materials Science: Materials in Electronics vol. 20 (SUPPL. 1)

DOI 10.1007/s10854-008-9740-z

01-01-2009

2008

Repair of thin thermally grown silicon dioxide by anodic oxidation
Chen L, Zhou Y, Krause S, Munoz AG, Kunze J and Schmuki P
Electrochimica Acta vol. 53 (8), 3395-3402.

DOI 10.1016/j.electacta.2007.11.065

10-03-2008

2007

Biosensor arrays based on the degradation of thin polymer films interrogated by scanning photoinduced impedance microscopy.
Zhou Y, Jiang S, Krause S and Chazalviel J-N
Anal Chem vol. 79 (23), 8974-8978.

DOI 10.1021/ac071437t

01-12-2007

Scanning photoinduced impedance microscopy using amorphous silicon photodiode structures.
Zhou Y, Chen L, Krause S and Chazalviel J-N
Anal Chem vol. 79 (16), 6208-6214.

DOI 10.1021/ac070797d

15-08-2007

2006

Scanning Photo-Induced Impedance Microscopy - Resolution studies and polymer characterization
Krause S, Moritz W, Talabani H, Xu M, Sabot A and Ensell G
Electrochimica Acta vol. 51 (8-9), 1423-1430.

DOI 10.1016/j.electacta.2005.02.119

QMRO

20-01-2006

Biosensor arrays based on the degradation of thin polymer films interrogated by Scanning Photo-induced Impedance Microscopy
Zhou YL, Krause S, Chazalviel JN and McNeil CJ
2006 IEEE SENSORS, VOLS 1-3., 259-262.

DOI 10.1109/ICSENS.2007.355769

01-01-2006

Sensors based on thin film degradation
KRAUSE SK, Fernandez-Sanchez C and McNeil CJ
In Encyclopedia of Sensors, American Scientific Publishers 289-306.
01-01-2006

2004

High resolution LAPS using amorphous silicon as the semiconductor material
Moritz W, Yoshinobu T, Finger F, Krause S, Martin-Fernandez M and Schoning MJ
Sensors and Actuators B-Chemical vol. 103 (1-2), 436-441.

DOI 10.1016/j.snb.2004.04.073

29-09-2004

2003

Impedance methods
KRAUSE SK
In Encyclopedia of Electrochemistry, Volume 3, Instrumentation and Electroanalytical Chemistry, Wiley-Vch Verlag 196-229.

DOI 10.1002/9783527610426.bard030206

01-01-2003

2002

Simultaneous quartz crystal microbalance impedance and electrochemical impedance measurements. Investigation into the degradation of thin polymer films.
Sabot A and Krause S
Anal Chem vol. 74 (14), 3304-3311.

DOI 10.1021/ac0200724

15-07-2002

Scanning photo-induced impedance microscopy - an impedance based imaging technique
Krause S, Talabani H, Xu M, Moritz W and Griffiths J
Electrochimica Acta vol. 47 (13-14), 2143-2148.

DOI 10.1016/S0013-4686(02)00088-9

25-05-2002

2001

Biosensor based on enzyme-catalysed degradation of thin polymer films
Sumner C, Krause S, Sabot A, Turner K and McNeil CJ
Biosensors & Bioelectronics vol. 16 (9-12), 709-714.

DOI 10.1016/s0956-5663(01)00210-x

01-12-2001

Re-activation of an all solid state oxygen sensor
Moritz W, Krause S, Roth U, Klimm D and Lippitz A
Anal Chim Acta vol. 437 (2), 183-190.

DOI 10.1016/S0003-2670(01)00993-X

27-06-2001

Combined QCM and electrochemical impedance measurements for biosensor applications.
KRAUSE SK, Sabot A and Sumner C
Chemical and Biological Sensors and Analytical Methods II, ECS Conference, San Francisco.
01-01-2001

The detection of organic pollutants in water with calixarene coated electrodes
Wilkop T, Krause S, Nabok A, Ray AK and Yates R
NOVEL METHODS TO STUDY INTERFACIAL LAYERS. vol. 11, 427-437. Editors: Moebius D and Miller R.

DOI 10.1016/S1383-7303(01)80037-5

01-01-2001

2000

A transducer based on enzyme-induced degradation of thin polymer films monitored by surface plasmon resonance.
Sumner C, Sabot A, Turner K and Krause S
Anal Chem vol. 72 (21), 5225-5232.

DOI 10.1021/ac000411y

01-11-2000

Photocurrent measurements for laterally resolved interface characterization
Moritz W, Gerhardt I, Roden D, Xu M and Krause S
Fresenius Journal of Analytical Chemistry vol. 367 (4), 329-333.

DOI 10.1007/s002160000409

01-06-2000

1999

Electrochemical sensor for measurement of urea and creatinine in serum based on ac impedance measurement of enzyme-catalyzed polymer transformation
Ho WO, Krause S, McNeil CJ, Pritchard JA, Armstrong RD, Athey D and Rawson K
Anal Chem vol. 71 (10), 1940-1946.

DOI 10.1021/ac981367d

15-05-1999

1998

Silicon-Based Sensor for Flourine Gas
Moritz W, Krause S, Bartholomäus L, Gabusjan T, Vasiliev AA, Godovski DY and Malyshev VV
Acs Symposium Series vol. 690, 119-129.

DOI 10.1021/bk-1998-0690.ch010

01-01-1998

1995

Electrochemical Sensors Based on Impedance Measurement of Enzyme-Catalyzed Polymer Dissolution: Theory and Applications
McNeil CJ, Athey D, Ball M, Ho WO, Krause S, Armstrong RD, Des Wright J and Rawson K
Analytical Chemistry, American Chemical Society (Acs) vol. 67 (21), 3928-3935.

DOI 10.1021/ac00117a018

01-11-1995

Monitoring of HF and F2 using a field-effect sensor
Moritz W, Krause S, Vasiliev AA, Godovski DY and Malyshev VV
Sensors and Actuators B Chemical, Elsevier vol. 24 (1-3), 194-196.

DOI 10.1016/0925-4005(95)85041-4

01-03-1995

1994

Improved long-term stability for an LaF3 based oxygen sensor
Krause S, Moritz W and Grohmann I
Sensors and Actuators B Chemical, Elsevier vol. 18 (1-3), 148-154.

DOI 10.1016/0925-4005(94)87074-8

01-03-1994

1992

A low-temperature oxygen sensor based on the Si/LaF3/Pt capacitive structure
Krause S, Moritz W and Grohmann I
Sensors and Actuators B Chemical, Elsevier vol. 9 (3), 191-196.

DOI 10.1016/0925-4005(92)80215-j

01-10-1992

1991

Chemical sensitivity of an ISFET with Ta2O5 membrane in strong acid and alkaline solutions
Bobrov PV, Tarantov YA, Krause S and Moritz W
Sensors and Actuators B Chemical, Elsevier vol. 3 (1), 75-81.

DOI 10.1016/0925-4005(91)85010-g

01-01-1991

Grants

Grants of specific relevance to the Centre for Bioengineering

3D Photoelectrochemical Imaging in Porous Light-Addressable Structures
Steffi Krause, Joe Briscoe and Thomas Iskratsch
£202,248 EPSRC Engineering and Physical Sciences Research Council
01-02-2021 - 30-04-2023

Materials development for carbon dioxide gas sensors
Steffi Krause and Joe Briscoe
£5,500 McGowan Sensor Labs Limited
10-09-2018 - 31-07-2019

Overseas Conference Travel Grant - Analytical Chemistry Trust Fund
Steffi Krause
£600 Analytical Chemistry Trust Fund
09-09-2018 - 12-09-2018

Combined LAPS and SICM for multimodal live cell imaging
Steffi Krause
£504,481 EPSRC Engineering and Physical Sciences Research Council
01-06-2018 - 28-02-2023

ZINCLAPS - Light-addressable potentiometric sensors (LAPS) for zinc imaging with high spatiotemporal resolution for elucidating the role of zinc in age related macular degeneration
Steffi Krause
£156,364 EU Commission - Horizon 2020
25-08-2017 - 24-08-2019