Research
ion channels, neuronal excitation, electrophysiology, biochemistry, sub-cellular protein trafficking
Interests
Calcium influx triggered by electrical excitation, regulated by Voltage-Activated Calcium Channels, is the fast and ubiquitous chemical signal in excitable tissues like the brain, heart, and muscle. Our group investigates the structure-function relationship, interactions, and cellular trafficking of neuronal and cerebrovascular Calcium Channel proteins. We use a combination of electrophysiology, flow cytometry, immunocytochemistry and biochemical techniques to investigate how the assembly of different protein subunits regulates the channels' biological activity and the mechanisms of action of drugs targeting them. We use both heterologous cell expression systems and primary neurons to investigate the channel function and cellular trafficking.
Publications
Publications of specific relevance to the Centre for Molecular Cell Biology
2022
ADAM17 Mediates Proteolytic Maturation of Voltage-Gated Calcium Channel Auxiliary α2δ Subunits, and Enables Calcium Current EnhancementKadurin I, Dahimene S, Page KM, Ellaway JIJ, Chaggar K, Troeberg L, Nagase H and Dolphin AC
Function,
Oxford University Press (Oup) vol. 3 (3)
17-03-2022Biallelic CACNA2D1 loss-of-function variants cause early-onset developmental epileptic encephalopathyDahimene S, von Elsner L, Holling T, Mattas LS, Pickard J, Lessel D, Pilch KS,
Kadurin I, Pratt WS, Zhulin IB, Dai H, Hempel M, Ruzhnikov MRZ, Kutsche K and Dolphin AC
Brain,
Oxford University Press (Oup) vol. 145 (8), 2721-2729.
16-03-2022 2020
Fight or flight: The culprit is lurking in the neighbourhoodDolphin AC and
Kadurin I Cell Calcium,
Elsevier vol. 87
11-02-2020 2019
Disruption of the Key Ca2+ Binding Site in the Selectivity Filter of Neuronal Voltage-Gated Calcium Channels Inhibits Channel TraffickingMeyer JO, Dahimene S, Page KM, Ferron L,
Kadurin I, Ellaway JIJ, Zhao P, Patel T, Rothwell SW, Lin P, Pratt WS and Dolphin AC
Cell Reports,
Elsevier vol. 29 (1), 22-33.e5.
01-10-2019 2018
The α2δ-like Protein Cachd1 Increases N-type Calcium Currents and Cell Surface Expression and Competes with α2δ-1Dahimene S, Page KM,
Kadurin I, Ferron L, Ho DY, Powell GT, Pratt WS, Wilson SW and Dolphin AC
Cell Reports,
Elsevier vol. 25 (6), 1610-1621.e5.
01-11-2018 Proteolytic maturation of α2δ controls the probability of synaptic vesicular releaseFerron L,
Kadurin I and Dolphin AC
Elife,
Elife vol. 7
19-06-2018 2017
LRP1 influences trafficking of N-type calcium channels via interaction with the auxiliary α2δ-1 subunitKadurin I, Rothwell SW, Lana B, Nieto-Rostro M and Dolphin AC
Scientific Reports,
Springer Nature vol. 7 (1)
03-03-2017 2016
Proteolytic maturation of α2δ represents a checkpoint for activation and neuronal trafficking of latent calcium channelsKadurin I, Ferron L, Rothwell SW, Meyer JO, Douglas LR, Bauer CS, Lana B, Margas W, Alexopoulos O, Nieto-Rostro M, Pratt WS and Dolphin AC
Elife,
Elife vol. 5
26-10-2016 Thrombospondin-4 reduces binding affinity of [3H]-gabapentin to calcium-channel α2δ-1-subunit but does not interact with α2δ-1 on the cell-surface when co-expressedLana B, Page KM,
Kadurin I, Ho S, Nieto-Rostro M and Dolphin AC
Scientific Reports,
Springer Nature vol. 6 (1)
14-04-2016 2014
Functional exofacially tagged N-type calcium channels elucidate the interaction with auxiliary α2δ-1 subunitsCassidy JS, Ferron L,
Kadurin I, Pratt WS and Dolphin AC
Proceedings of The National Academy of Sciences of The United States of America,
Proceedings of The National Academy of Sciences vol. 111 (24), 8979-8984.
02-06-2014 The inhibition of functional expression of calcium channels by prion protein demonstrates competition with α2δ for GPI-anchoring pathwaysAlvarez-Laviada A,
Kadurin I, Senatore A, Chiesa R and Dolphin AC
Biochemical Journal,
Portland Press vol. 458 (2), 365-374.
14-02-2014 2012
Calcium Currents Are Enhanced by α2δ-1 Lacking Its Membrane Anchor*Kadurin I, Alvarez-Laviada A, Ng SFJ, Walker-Gray R, D'Arco M, Fadel MG, Pratt WS and Dolphin AC
Journal of Biological Chemistry,
Elsevier vol. 287 (40), 33554-33566.
06-08-2012 Voltage-Gated Calcium Channel α2δ Subunits in Lipid Rafts: The Importance of Proteolytic Cleavage Into α2 and δKadurin I, Bauer C, Lana B, Alvarez-Laviada A, Nieto-Rostro M, Douglas L, Troeberg L, Nagase H and Dolphin AC
Biophysical Journal. vol. 102 (3)
01-01-2012 2011
Presynaptic HCN1 channels regulate CaV3.2 activity and neurotransmission at select cortical synapsesHuang Z, Lujan R,
Kadurin I, Uebele VN, Renger JJ, Dolphin AC and Shah MM
Nature Neuroscience,
Springer Nature vol. 14 (4), 478-486.
27-02-2011 2010
A new look at calcium channel α2δ subunitsBauer CS, Tran-Van-Minh A,
Kadurin I and Dolphin AC
Current Opinion in Neurobiology,
Elsevier vol. 20 (5), 563-571.
25-06-2010 The α2δ subunits of voltage-gated calcium channels form GPI-anchored proteins, a posttranslational modification essential for functionDavies A,
Kadurin I, Alvarez-Laviada A, Douglas L, Nieto-Rostro M, Bauer CS, Pratt WS and Dolphin AC
Proceedings of The National Academy of Sciences of The United States of America,
Proceedings of The National Academy of Sciences vol. 107 (4), 1654-1659.
04-01-2010 2009
The Increased Trafficking of the Calcium Channel Subunit α2δ-1 to Presynaptic Terminals in Neuropathic Pain Is Inhibited by the α2δ Ligand PregabalinBauer CS, Nieto-Rostro M, Rahman W, Tran-Van-Minh A, Ferron L, Douglas L,
Kadurin I, Ranjan YS, Fernandez-Alacid L, Millar NS, Dickenson AH, Lujan R and Dolphin AC
Journal of Neuroscience,
Society For Neuroscience vol. 29 (13), 4076-4088.
01-04-2009 A single conserved proline residue determines the membrane topology of stomatinKadurin I, Huber S and Gründer S
Biochemical Journal,
Portland Press vol. 418 (3), 587-594.
25-02-2009 2008
Differential effects of N-glycans on surface expression suggest structural differences between the acid-sensing ion channel (ASIC) 1a and ASIC1bKadurin I, Golubovic A, Leisle L, Schindelin H and Gründer S
Biochemical Journal,
Portland Press vol. 412 (3), 469-475.
28-05-2008 2007
Zebrafish Acid-sensing Ion Channel (ASIC) 4, Characterization of Homo- and Heteromeric Channels, and Identification of Regions Important for Activation by H+ *Chen X, Polleichtner G,
Kadurin I and Gründer S
Journal of Biological Chemistry,
Elsevier vol. 282 (42), 30406-30413.
07-08-2007 2006
Strong modulation by RFamide neuropeptides of the ASIC1b/3 heteromer in competition with extracellular calciumChen X, Paukert M,
Kadurin I, Pusch M and Gründer S
Neuropharmacology,
Elsevier vol. 50 (8), 964-974.
20-03-2006
